Why should we learn this stuff? After all, nobody solves structures with UV any longer!
* Many organic molecules have chromophores that absorb UV
* UV absorbance is about 1000 x easier to detect per mole than NMR
* Still used in following reactions where the chromophore changes. Useful because timescale is so fast, and sensitivity so high. Kinetics, esp. in biochemistry, enzymology.
* Most quantitative Analytical chemistry in organic chemistry is conducted using HPLC with UV detectors
* One wavelength may not be the best for all compound in a mixture.
* Affects quantitative interpretation of HPLC peak heights.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment